resE [2019-08-07 08:55:50]

two-component sensor kinase, regulation of aerobic and anaerobic respiration

locus: BSU_23110

pI: 5.34

mw: 66.60 kDa

protein length: 589 aa

gene length: 1770 bp

function: regulation of aerobic and anaerobic respiration

product: two-component sensor kinase

essential: no

synonyms: ypxE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.1|Regulators of electron transport]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 2,415,415 → 2,417,184

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]

Paralogous protein(s)

• [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR], [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK]

[SW|Domains]

• two transmembrane segments
• [SW|PAS domain]
• C-terminal histidine phosphotransferase domain

Modification

• autophosphorylation on a His residue

Structure

• [PDB|4ZR7] (N-terminal domain)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|22383849]

• genes: [gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|8631715]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8631715], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [PubMed|8631715,11222591], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|9988472], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16825793], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]

• genes: [gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|8631715]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]

Biological materials

Mutant

• BKE23110 (Δ[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTACTACGCTTTTCCAAA, downstream forward: _UP4_TAAAATCGAGTCTGAATTTG
• BKK23110 (Δ[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTACTACGCTTTTCCAAA, downstream forward: _UP4_TAAAATCGAGTCTGAATTTG

References

Reviews

• 23046954

Original publications

• 16885456,8631715,10094672,8682783,9352926,8631715,11222591,9988472,16825793,26297017,25909364,28899391