resE [2019-08-07 08:55:50]

two-component sensor kinase, regulation of aerobic and anaerobic respiration

Locus: BSU_23110

Isoelectric point: 5.34

Molecular weight: 66.60 kDa

Protein length: 589 aa

Gene length: 1770 bp

Function: regulation of aerobic and anaerobic respiration

Product: two-component sensor kinase

Essential: no

Synonyms: ypxE

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Regulators of electron transport
• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Two-component sensor kinase
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Gene

Coordinates: 2,415,415 → 2,417,184

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of ResD

Paralogous protein(s)

• PhoR, WalK

Domains

• two transmembrane segments
• PAS domain
• C-terminal histidine phosphotransferase domain

Modification

• autophosphorylation on a His residue

Structure

• 4ZR7 (N-terminal domain)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: rluB-resA-resB-resC-resD-resE
  Description: PubMed

• Genes: resA-resB-resC-resD-resE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • PhoP: activation, PubMed, in PhoP regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

• Genes: resD-resE
  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE23110 (ΔresE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCCTACTACGCTTTTCCAAA, downstream forward: _UP4_TAAAATCGAGTCTGAATTTG
• BKK23110 (ΔresE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCCTACTACGCTTTTCCAAA, downstream forward: _UP4_TAAAATCGAGTCTGAATTTG

References

Reviews

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Original publications

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